pCDH-puro-Bcl-XL
(Plasmid #46972)

• Purpose: lentiviral expression of Bcl-XL
• Depositing Lab: Jialiang Wang
• Publication: Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.

Backbone

• Vector backbone: PCDH-CMV-MCS-EF1-PURO
• Backbone manufacturer: System Biosciences
• Total vector size (bp): 7384
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BCL2L1
• Alt name: Bcl-XL
• Alt name: BCL2-like 1
• Alt name: BCLX
• Species: H. sapiens (human)
• Insert Size (bp): 700
• Entrez Gene: BCL2L1 (a.k.a. BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bcl-xL was cut from 3120 pSFFV-neo Bcl-XL(Addgene #8749) and subcloned into the EcoRI sites of pCDH-CMV-MCS-EF1-Puro.