pLenti CMV MCL1 Puro
(Plasmid #140746)

• Purpose: Expresses MCL1 in mammalian cells
• Depositing Lab: Scott Dixon
• Publication: Inde et al Cell Rep. 2020 Jul 7;32(1):107845. doi: 10.1016/j.celrep.2020.107845.

Backbone

• Vector backbone: pLenti CMV Puro DEST (w118-1)
• Backbone manufacturer: 17452
• Backbone size w/o insert (bp): 9628
• Total vector size (bp): 9023
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MCL1
• Species: H. sapiens (human)
• Insert Size (bp): 1076
• GenBank ID: NM_021960.5
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pLenti CMV MCL1 STOP Puro.gb
• A portion of this plasmid was derived from a plasmid made by: Dr. Aaron Gitler, Stanford University
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry