pEGFP-N1-RACK1
(Plasmid #41088)

• Depositing Lab: Anna Huttenlocher
• Depositing Organization: University of Wisconsin, Madison
  Located in United States of America
• Publication: Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.
• Citations: 8 Articles

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 5669
• Modifications to backbone: PCR-amplified insert was blunt-end ligated into pPCR-Script AMP vector, removed by digestion with HindIII and KpnI, and ligated into pEGFP-N1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Receptor for activated C kinase
• Alt name: RACK1
• Alt name: GNB2L1
• Species: H. sapiens (human)
• Insert Size (bp): 954
• GenBank ID: NM_006098.4
• Entrez Gene: RACK1 (a.k.a. GNB2L1, Gnb2-rs1, H12.3, HLC-7, PIG21)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP_C_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pcDNA3.1 RACK1 was recieved from T.E. O'Toole at Scripps Research Institute then cloned into N1-pEGFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 41088-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 41088-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.