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pcDNA3.1-ZNF598-TEV-3xFLAG
(Plasmid #105690)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105690 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5059
  • Total vector size (bp) 7768
  • Modifications to backbone
    TEV-3xFLAG was inserted into the vector to serve as a C-terminal tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZNF598
  • Alt name
    zinc finger protein 598
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2717
  • GenBank ID
    NM_178167
  • Entrez Gene
    ZNF598 (a.k.a. HEL2)
  • Promoter CMV (from vector)
  • Tag / Fusion Protein
    • TEV-3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer GGTCTATATAAGCAGAGC
  • 3′ sequencing primer GCCTGCTATTGTCTTCCC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ZNF598 CDA was originally cloned from the origene plasmid RC208429.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-ZNF598-TEV-3xFLAG was a gift from Ramanujan Hegde (Addgene plasmid # 105690 ; http://n2t.net/addgene:105690 ; RRID:Addgene_105690)
  • For your References section:

    Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination. Juszkiewicz S, Hegde RS. Mol Cell. 2017 Feb 16;65(4):743-750.e4. doi: 10.1016/j.molcel.2016.11.039. Epub 2017 Jan 5. 10.1016/j.molcel.2016.11.039 PubMed 28065601