Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells.
Agrotis A, Pengo N, Burden JJ, Ketteler R
Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122842pDEST-CMV-N-EGFPN-terminal EGFP tag. Gateway destination vector for mammalian expression.
Loading...
122843pDEST-CMV-polysiteGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
Loading...
122844pDEST-CMV-C-EGFPC-terminal EGFP tag. Gateway destination vector for mammalian expression.
Loading...
122845pDEST-CMV-3xFLAG-gateway-EGFPN-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.
Loading...
122848pLVpuro-CMV-N-EGFPN-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.
Loading...
123069pDONR223 LC3A WTGateway entry clone encoding human MAP1LC3A wild-type
Loading...
123070pDONR223 LC3A G120AGateway entry clone encoding human MAP1LC3A G120A
Loading...
123071pDONR223 LC3A G120Gateway entry clone encoding human MAP1LC3A G120
Loading...
123072pDONR223 LC3B WTGateway entry clone encoding human MAP1LC3B wild-type
Loading...
123073pDONR223 LC3B G120AGateway entry clone encoding human MAP1LC3B G120A
Loading...
123074pDONR223 LC3B G120Gateway entry clone encoding human MAP1LC3B G120
Loading...
123075pDONR223 LC3B2 WTGateway entry clone encoding human MAP1LC3B2 wild-type
Loading...
123076pDONR223 LC3B2 G120AGateway entry clone encoding human MAP1LC3B2 G120A
Loading...
123077pDONR223 LC3B2 G120Gateway entry clone encoding human MAP1LC3B2 G120
Loading...
123078pDONR223 LC3C WTGateway entry clone encoding human MAP1LC3C wild-type
Loading...
123079pDONR223 LC3C G126Gateway entry clone encoding human MAP1LC3C G126
Loading...
123080pDONR223 GABARAP WTGateway entry clone encoding human GABARAP wild-type
Loading...
123081pDONR223 GABARAP G116AGateway entry clone encoding human GABARAP G116A
Loading...
123082pDONR223 GABARAP G116Gateway entry clone encoding human GABARAP G116
Loading...
123083pDONR223 GABARAPL1 WTGateway entry clone encoding human GABARAPL1 wild-type
Loading...
123084pDONR223 GABARAPL1 G116AGateway entry clone encoding human GABARAPL1 G116A
Loading...
123085pDONR223 GABARAPL1 G116Gateway entry clone encoding human GABARAPL1 G116
Loading...
123086pDONR223 GABARAPL2 WTGateway entry clone encoding human GABARAPL2 wild-type
Loading...
123087pDONR223 GABARAPL2 G116AGateway entry clone encoding human GABARAPL2 G116A
Loading...
123088pDONR223 GABARAPL2 G116Gateway entry clone encoding human GABARAPL2 G116
Loading...
123089pCMV 3xFLAG-LC3A WTExpresses 3xFLAG-LC3A wild-type in mammalian cells.
Loading...
123090pCMV 3xFLAG-LC3A G120AExpresses 3xFLAG-LC3A G120A in mammalian cells.
Loading...
123091pCMV 3xFLAG-LC3A G120Expresses 3xFLAG-LC3A G120 in mammalian cells.
Loading...
123092pCMV 3xFLAG-LC3B WTExpresses 3xFLAG-LC3B wild-type in mammalian cells.
Loading...
123093pCMV 3xFLAG-LC3B G120AExpresses 3xFLAG-LC3B G120A in mammalian cells.
Loading...
123094pCMV 3xFLAG-LC3B G120Expresses 3xFLAG-LC3B G120 in mammalian cells.
Loading...
123095pCMV 3xFLAG-LC3C WTExpresses 3xFLAG-LC3C wild-type in mammalian cells.
Loading...
123096pCMV 3xFLAG-LC3C G126Expresses 3xFLAG-LC3C G126 in mammalian cells.
Loading...
123097pCMV 3xFLAG-GABARAP WTExpresses 3xFLAG-GABARAP wild-type in mammalian cells.
Loading...
123098pCMV 3xFLAG-GABARAP G116AExpresses 3xFLAG-GABARAP G116A in mammalian cells.
Loading...
123099pCMV 3xFLAG-GABARAP G116Expresses 3xFLAG-GABARAP G116 in mammalian cells.
Loading...
123100pCMV 3xFLAG-GABARAPL1 WTExpresses 3xFLAG-GABARAPL1 wild-type in mammalian cells.
Loading...
123101pCMV 3xFLAG-GABARAPL1 G116AExpresses 3xFLAG-GABARAPL1 G116A in mammalian cells.
Loading...
123102pCMV 3xFLAG-GABARAPL1 G116Expresses 3xFLAG-GABARAPL1 G116 in mammalian cells.
Loading...
123103pCMV 3xFLAG-GABARAPL2 WTExpresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.
Loading...
123104pCMV 3xFLAG-GABARAPL2 G116AExpresses 3xFLAG-GABARAPL2 G116A in mammalian cells.
Loading...
123105pCMV 3xFLAG-GABARAPL2 G116Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.
Loading...
123106pDEST-CMV 3xFLAG-LC3A-GFPExpresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A
Loading...
123107pDEST-CMV 3xFLAG-LC3B-GFPExpresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B
Loading...
123108pDEST-CMV 3xFLAG-LC3B-G120A-GFPExpresses 3xFLAG-LC3B-G120A-EGFP in mammalian cells, negative control for monitoring ATG4 activity.
Loading...
123109pDEST-CMV 3xFLAG-LC3B2-GFPExpresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2
Loading...
123110pDEST-CMV 3xFLAG-LC3C-GFPExpresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C
Loading...
123111pDEST-CMV 3xFLAG-GABARAP-GFPExpresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP
Loading...
123112pDEST-CMV 3xFLAG-GABARAPL1-GFPExpresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1
Loading...
123113pDEST-CMV 3xFLAG-GABARAPL2-GFPExpresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2
Loading...
123114pDEST-CMV GFP-LC3B WTExpresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.
Loading...
123115pDEST-CMV GFP-LC3B G120AExpresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.
Loading...
123116pDEST-CMV GFP-LC3B G120Expresses EGFP-LC3B G120 in mammalian cells.
Loading...
123117pDEST-CMV GFP-GABARAPL1 WTExpresses EGFP-GABARAPL1 wild-type in mammalian cells.
Loading...
123118pDEST-CMV GFP-GABARAPL1 G116AExpresses EGFP-GABARAPL1 G116A in mammalian cells.
Loading...
123119pDEST-CMV GFP-GABARAPL1 G116Expresses EGFP-GABARAPL1 G116 in mammalian cells.
Loading...
123204pDONR223 LC3A no-stopGateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging
Loading...
123205pDONR223 LC3B no-stopGateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging
Loading...
123206pDONR223 LC3B G120A no-stopGateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging
Loading...
123207pDONR223 LC3B2 no-stopGateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging
Loading...
123208pDONR223 LC3C no-stopGateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging
Loading...
123209pDONR223 GABARAP no-stopGateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging
Loading...
123210pDONR223 GABARAPL1 no-stopGateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging
Loading...
123211pDONR223 GABARAPL2 no-stopGateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging
Loading...
123215pDEST-CMV-N-mCherryN-terminal mCherry tag. Gateway destination vector for mammalian expression.
Loading...
123216pDEST-CMV-N-Tandem-mCherry-EGFPN-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.
Loading...
123221pLVpuro-CMV-N-mCherryN-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.
Loading...
123223pLVpuro-CMV-N-3xFLAGN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.
Loading...
123230pDEST-CMV mCherry-GFP-LC3B WTExpresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.
Loading...
123235pDEST-CMV mCherry-GFP-LC3B G120AExpresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.
Loading...
123241pLenti PGK Puro GFP-LC3B G120Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.
Loading...
123242pLenti PGK Puro GFP-LC3B G120AExpression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
Loading...
123243pLenti PGK Puro GFP-GABARAPL1 G116Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.
Loading...
123245pLenti PGK Puro GFP-GABARAPL1 G116AExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.
Loading...