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Addgene

pDEST-CMV-N-mCherry
(Plasmid #123215)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123215 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA-DEST53
  • Backbone manufacturer
    Life Technologies
  • Backbone size (bp) 7720
  • Modifications to backbone
    Replacement of cycle3 GFP with mCherry between SacI restriction sites, loss of T7 promoter.
  • Vector type
    Mammalian Expression ; Gateway destination
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-CMV-N-mCherry was a gift from Robin Ketteler (Addgene plasmid # 123215 ; http://n2t.net/addgene:123215 ; RRID:Addgene_123215)
  • For your References section:

    Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Agrotis A, Pengo N, Burden JJ, Ketteler R. Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925. 10.1080/15548627.2019.1569925 PubMed 30661429