|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31907||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4722
Modifications to backboneGateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
Vector typeMammalian Expression
- Promoter CMV
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Please note: the orientation of the MCS within this plasmid places mCherry on the c-terminus of your gene of interest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDest-mCherry-N1 was a gift from Robin Shaw (Addgene plasmid # 31907 ; http://n2t.net/addgene:31907 ; RRID:Addgene_31907)
For your References section:BIN1 localizes the L-type calcium channel to cardiac T-tubules. Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, Colecraft HM, Shaw RM. PLoS Biol. 2010 Feb . 8(2):e1000312. 10.1371/journal.pbio.1000312 PubMed 20169111