|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18700||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6932
Modifications to backboneGateway cassette was inserted into original vector to create the pEZYflag Gateway Destination vector
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsE.coli strain DB3.1 @ 30C
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3') (Common Sequencing Primers)
pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEZYflag was a gift from Yu-Zhu Zhang (Addgene plasmid # 18700 ; http://n2t.net/addgene:18700 ; RRID:Addgene_18700)
For your References section:An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608