An in vitro recombination method to convert restriction- and ligation-independent expression vectors.
Guo F, Chiang MY, Wang Y, Zhang YZ
Biotechnol J. 2008 Mar . 3(3):370-7. PubMed Article
Guo F, Chiang MY, Wang Y, Zhang YZ
Biotechnol J. 2008 Mar . 3(3):370-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 18667 | pBZ-attBtxnl4b | cloning vector containing attB flanked TXNL4B useful for recombination based conversion of conventional vectors to Gateway compatible destination vectors |
| 18668 | pEZY19 | pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site. |
| 18669 | pEZYHb | pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria |
| 18671 | pEZYegfp | pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells |
| 18672 | pEZY3 | pcDNA3 converted Gateway Destination vector for mammalian expression |
| 18700 | pEZYflag | pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells |
| 18701 | pEZYmyc-His | pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells |
| 18703 | pEZYshuttle | pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system |
| 18704 | pEZY45 | pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector |
| 18705 | pEZY202 | pEG202 converted Gateway Destination vector for yeast two-hybrid bait vector |
| 18707 | pEZYpci | pPIC3.5K converted Gateway Destionation vector for multiple copy integration of gene of interest into Pichia |