|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18668||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7934
Modifications to backboneGateway cassette was inserted into original vector to create the pEZY19 Gateway Destination vector
Vector typeBacterial Expression ; Gateway Destination vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsE.coli strain DB3.1 @ 30C
- Promoter T7
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEZY19 was a gift from Yu-Zhu Zhang (Addgene plasmid # 18668 ; http://n2t.net/addgene:18668 ; RRID:Addgene_18668)
For your References section:An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608