Purpose(Empty Backbone) Gateway destination vector for bacterial expression; His tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11518||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8266
Vector typeBacterial Expression ; Gateway Destination Vector
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsMust be grown in a strain resistant to ccdB (DB3.1, ccdB Survival, or F').
Copy numberLow Copy
/ Fusion Protein
- His6 (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDest-527 was a gift from Dominic Esposito (Addgene plasmid # 11518 ; http://n2t.net/addgene:11518 ; RRID:Addgene_11518)