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pDEST-periHisMBP
(Plasmid #11086)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 11086 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMal-C2
  • Backbone manufacturer
    New England Biolabs
  • Backbone size (bp) 6000
  • Vector type
    Bacterial Expression
  • Promoter tac promoter
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • MBP (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    plasmid must be grown in gyrA mutant resistant to the toxin CcdB
  • Copy number
    Low Copy

Cloning Information

Resource Information

Depositor Comments

The MBP ORF can be sequenced using 5'-GAG CGG ATA ACA ATT TCA CAC AGG-3' as a tac promoter sequencing primer (in the forward direction). To sequence inserts fused to the C-terminus of MBP, the primers are 5'-GAT GAA GCC CTG AAA GAC GCG CAG-3' (forward) and 5'-GCA AGG CGA TTA AGT TGG GTA ACG C-3' (reverse).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-periHisMBP was a gift from David Waugh (Addgene plasmid # 11086 ; http://n2t.net/addgene:11086 ; RRID:Addgene_11086)
  • For your References section:

    Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Nallamsetty S, Austin BP, Penrose KJ, Waugh DS. Protein Sci. 2005 Dec . 14(12):2964-71. 10.1110/ps.051718605 PubMed 16322578