|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18707||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 11282
Modifications to backboneGateway cassette was inserted into original vector to create the pEZYpci Gateway Destination vector
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth instructionsE.coli strain DB3.1 @ 30C
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEZYpci was a gift from Yu-Zhu Zhang (Addgene plasmid # 18707 ; http://n2t.net/addgene:18707 ; RRID:Addgene_18707)
For your References section:An in vitro recombination method to convert restriction- and ligation-independent expression vectors. Guo F, Chiang MY, Wang Y, Zhang YZ. Biotechnol J. 2008 Mar . 3(3):370-7. 10.1002/biot.200700170 PubMed 18064608