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pDest-eGFP-N1
(Plasmid #31796)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31796 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pegfp-N1
  • Backbone manufacturer
    Clontech
  • Backbone size (bp) 4722
  • Modifications to backbone
    Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest-eGFP-N1 was a gift from Robin Shaw (Addgene plasmid # 31796 ; http://n2t.net/addgene:31796 ; RRID:Addgene_31796)
  • For your References section:

    BIN1 localizes the L-type calcium channel to cardiac T-tubules. Hong TT, Smyth JW, Gao D, Chu KY, Vogan JM, Fong TS, Jensen BC, Colecraft HM, Shaw RM. PLoS Biol. 2010 Feb . 8(2):e1000312. 10.1371/journal.pbio.1000312 PubMed 20169111