178,379 results
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Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pGP-AAV-syn-jGCaMP8s-WPRE
Plasmid#162374PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas serviceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
TagsV5ExpressionMammalianPromoterTRE3GAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro
Plasmid#141370PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Cbh_v5 AAV-ABE C-terminal
Plasmid#137178PurposeAAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCMVAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV Retrograde)
Viral prep#112677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV Retrograde trial size)
Viral prep#37825-AAVrg.TPurposeReady-to-use AAV Retrograde trial size particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. GFP expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mTagBFP2
Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.TBG.PI.eGFP.WPRE.bGH
Plasmid#105535PurposeAAV expression of EGFP from TBG promoterDepositorHas serviceAAV8InsertEGFP
UseAAVExpressionMammalianPromoterTBGAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-jGCaMP7b
Plasmid#104484PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7b
TagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mTH-GFP
Plasmid#99128PurposeAn AAV genome that expresses GFP from the mouse TH promoterDepositorInsertGFP
UseAAVExpressionMammalianPromotermTHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAnc80L65AAP
Plasmid#92307PurposeAdeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAPDepositorInsertAncestral AAV Capsid Anc80L65AAP
UseAAV and Synthetic BiologyExpressionMammalianPromoterRep2Available sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
TagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterMaize ubiquitin 1 (ZmUbi1)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIP-CMV-tagRFP-FLAG-cGAS
Plasmid#86676PurposeExpresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInserttagRFP-FLAG-cGAS
UseLentiviralAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-PDi-CRISPRn
Plasmid#73500PurposeDox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locusDepositorInsertsCas9
rtTA
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCAG and TREAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.2 TDP-43 YFP
Plasmid#84911Purposeexpression of TDP-43 YFP in mammalian cellsDepositorAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralExpressionMammalianAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-his-LbCpf1-EC
Plasmid#79008PurposeBacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)DepositorInsertLbCpf1
TagsMBP-6xHis-NLSExpressionBacterialPromotertac promoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by