In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.
Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. PubMed Article
Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC
Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 87108 | pCAG-1BPNLS-Cas9-1BPNLS | BPNLS-Cas9-BPNLS expression plasmid |
| 87109 | pCAG-1BPNLS-Cas9-1BPNLS-2AGFP | BPNLS-Cas9-BPNLS-2AGFP expression plasmid |
| 87110 | pCAGmCherry-gRNA | gRNA cloning vector with CAGmCherry |
| 87111 | Tubb3gRNA-mCherry | mouse Tubb3 target gRNA expression plasmid with CAGmCherry |
| 87112 | pTubb3-MC | minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI |
| 87113 | pAi14-luc-MC | minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI |
| 87114 | pAi14-GFPNLS-MC | minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI |
| 87115 | pAAV-nEFCas9 | SpCas9 expression AAV backbone plasmid under control of nEF promoter |
| 87116 | pAAV-mTubb3 | AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI |
| 87117 | pAAV-Ai14-HITI | AAV backbone plasmid including GFPNLS-pA knock-in donor and Ai14gRNA for HITI |
| 87118 | pAAV-Ai14-luc | AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI |
| 87119 | pAAV-rMERTK-HITI | Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI |