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Versatile protein tagging in cells with split fluorescent protein.
Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
70217pEGFP-GFP11-Clathrin light chainExpresses GFP11-clathrin light chain in mammalian cells
70218pACUH-GFP11x7-mCherry-α-tubulinExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.
70219pcDNA3.1-GFP(1-10)Expresses GFP(1-10) in mammalian cells
70220pEGFP-sfCherry11x4-ß-actinExpresses sfCherry11x4-ß-actin in mammalian cells
70221pEGFP-sfCherry11-ß-actinExpresses sfCherry11-ß-actin in mammalian cells
70222pcDNA3.1-sfCherry(1-10)Expresses sfCherry(1-10) in mammalian cells
70224pHRm-NLS-dCas9-GFP11x7-NLSExpresses NLS-dCas9-GFP11x7-NLS in mammalian cells
70228pHRm-GFP(1-10)-VP64-NLSExpresses GFP(1-10)-VP64-NLS in mammalian cells
80409pHR-SFFV-GFP1-10Expresses GFP1-10 in mammalian cells

Antibodies from Article