Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

PU6::unc-119_sgRNA Citations (25)

Originally described in: Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco JA Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532. PubMed Journal

Articles Citing PU6::unc-119_sgRNA

Articles
CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. Katic I, Xu L, Ciosk R. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. PubMed

Associated Plasmids
REC-1 and HIM-5 distribute meiotic crossovers and function redundantly in meiotic double-strand break formation in Caenorhabditis elegans. Chung G, Rose AM, Petalcorin MI, Martin JS, Kessler Z, Sanchez-Pulido L, Ponting CP, Yanowitz JL, Boulton SJ. Genes Dev. 2015 Sep 15;29(18):1969-79. doi: 10.1101/gad.266056.115. PubMed
TMEM107 recruits ciliopathy proteins to subdomains of the ciliary transition zone and causes Joubert syndrome. Lambacher NJ, Bruel AL, van Dam TJ, Szymanska K, Slaats GG, Kuhns S, McManus GJ, Kennedy JE, Gaff K, Wu KM, van der Lee R, Burglen L, Doummar D, Riviere JB, Faivre L, Attie-Bitach T, Saunier S, Curd A, Peckham M, Giles RH, Johnson CA, Huynen MA, Thauvin-Robinet C, Blacque OE. Nat Cell Biol. 2016 Jan;18(1):122-31. doi: 10.1038/ncb3273. Epub 2015 Nov 23. PubMed
SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. PubMed

Associated Plasmids
Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs. Choi YS, Edwards LO, DiBello A, Jose AM. Nucleic Acids Res. 2017 Jun 2;45(10):e87. doi: 10.1093/nar/gkx091. PubMed
The double-stranded RNA binding protein RDE-4 can act cell autonomously during feeding RNAi in C. elegans. Raman P, Zaghab SM, Traver EC, Jose AM. Nucleic Acids Res. 2017 May 24. doi: 10.1093/nar/gkx484. PubMed
The secreted MSP domain of C. elegans VAPB homolog VPR-1 patterns the adult striated muscle mitochondrial reticulum via SMN-1. Schultz J, Lee SJ, Cole T, Hoang HD, Vibbert J, Cottee PA, Miller MA, Han SM. Development. 2017 Jun 15;144(12):2175-2186. doi: 10.1242/dev.152025. PubMed
The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development. Cottee PA, Cole T, Schultz J, Hoang HD, Vibbert J, Han SM, Miller MA. Development. 2017 Jun 15;144(12):2187-2199. doi: 10.1242/dev.152207. PubMed
Caenorhabditis elegans ATPase inhibitor factor 1 (IF1) MAI-2 preserves the mitochondrial membrane potential (Deltapsim) and is important to induce germ cell apoptosis. Fernandez-Cardenas LP, Villanueva-Chimal E, Salinas LS, Jose-Nunez C, Tuena de Gomez Puyou M, Navarro RE. PLoS One. 2017 Aug 22;12(8):e0181984. doi: 10.1371/journal.pone.0181984. eCollection 2017. PubMed
AIP limits neurotransmitter release by inhibiting calcium bursts from the ryanodine receptor. Chen B, Liu P, Hujber EJ, Li Y, Jorgensen EM, Wang ZW. Nat Commun. 2017 Nov 9;8(1):1380. doi: 10.1038/s41467-017-01704-z. PubMed
Distinct regions of the intrinsically disordered protein MUT-16 mediate assembly of a small RNA amplification complex and promote phase separation of Mutator foci. Uebel CJ, Anderson DC, Mandarino LM, Manage KI, Aynaszyan S, Phillips CM. PLoS Genet. 2018 Jul 23;14(7):e1007542. doi: 10.1371/journal.pgen.1007542. eCollection 2018 Jul. PubMed
Rap2 and TNIK control Plexin-dependent tiled synaptic innervation in C. elegans. Chen X, Shibata AC, Hendi A, Kurashina M, Fortes E, Weilinger NL, MacVicar BA, Murakoshi H, Mizumoto K. Elife. 2018 Jul 31;7. pii: 38801. doi: 10.7554/eLife.38801. PubMed
SLO potassium channels antagonize premature decision making in C. elegans. Aoki I, Tateyama M, Shimomura T, Ihara K, Kubo Y, Nakano S, Mori I. Commun Biol. 2018 Aug 24;1:123. doi: 10.1038/s42003-018-0124-5. eCollection 2018. PubMed
Differential Requirements for Centrioles in Mitotic Centrosome Growth and Maintenance. Cabral G, Laos T, Dumont J, Dammermann A. Dev Cell. 2019 Aug 5;50(3):355-366.e6. doi: 10.1016/j.devcel.2019.06.004. Epub 2019 Jul 11. PubMed
Genome editing in the nematode Caenorhabditis briggsae using the CRISPR/Cas9 system. Culp E, Richman C, Sharanya D, Jhaveri N, van den Berg W, Gupta BP. Biol Methods Protoc. 2020 Feb 10;5(1):bpaa003. doi: 10.1093/biomethods/bpaa003. eCollection 2020. PubMed
New allele of C. elegans gene pign-1, named as xyz11. Narimatsu T, Ihara S. MicroPubl Biol. 2019 Jan 18;2019. doi: 10.17912/micropub.biology.000088. PubMed
The type II integral ER membrane protein VAP-B homolog in C. elegans is cleaved to release the N-terminal MSP domain to signal non-cell-autonomously. Zein-Sabatto H, Cole T, Hoang HD, Tiwary E, Chang C, Miller MA. Dev Biol. 2021 Feb;470:10-20. doi: 10.1016/j.ydbio.2020.10.015. Epub 2020 Nov 5. PubMed
IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans. Kuo CJ, Hsu YC, Wang ST, Liou BY, Lim SB, Chen YW, Chen CS. Front Immunol. 2020 Nov 30;11:561337. doi: 10.3389/fimmu.2020.561337. eCollection 2020. PubMed
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. PubMed

Associated Plasmids
N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans. Ihara S. MicroPubl Biol. 2021 Apr 29;2021. doi: 10.17912/micropub.biology.000386. PubMed
Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line. Singh R, Smit RB, Wang X, Wang C, Racher H, Hansen D. PLoS Genet. 2021 Sep 23;17(9):e1009687. doi: 10.1371/journal.pgen.1009687. eCollection 2021 Sep. PubMed
DREAM interrupted: severing LIN-35-MuvB association in Caenorhabditis elegans impairs DREAM function but not its chromatin localization. Goetsch PD, Strome S. Genetics. 2022 Jul 4;221(3). pii: 6584818. doi: 10.1093/genetics/iyac073. PubMed
New alleles of the SWI/SNF chromatin remodeling complex gene phf-10. Mathies LD, Blackwell G, Bettinger JC. MicroPubl Biol. 2022 Mar 17;2022. doi: 10.17912/micropub.biology.000533. eCollection 2022. PubMed
Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. Wu Y, Ding C, Weinreb A, Manning L, Swaim G, Yogev S, Colon-Ramos DA, Hammarlund M. bioRxiv. 2023 Jul 12:2023.07.12.548706. doi: 10.1101/2023.07.12.548706. Preprint. PubMed
PBRM-1/PBAF-regulated genes in a multipotent progenitor in Caenorhabditis elegans. Mathies LD, Kim AC, Soukup EM, Thomas AE, Bettinger JC. G3 (Bethesda). 2024 Mar 6;14(3):jkad297. doi: 10.1093/g3journal/jkad297. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.