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Addgene

pMLS260
(Plasmid #73685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ150
  • Vector type
    Worm Expression ; FLP/FRT
  • Selectable markers
    Cbr-unc-119

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
  • Species
    C. elegans (nematode), S. cerevisiae (budding yeast)
  • Mutation
    G5D
  • Promoter Punc-17

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 Reverse
  • 3′ sequencing primer M13 (-21) Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMLS260 was a gift from Erik Jorgensen (Addgene plasmid # 73685 ; http://n2t.net/addgene:73685 ; RRID:Addgene_73685)

  • For your References section:

    SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans. Schwartz ML, Jorgensen EM. Genetics. 2016 Feb 2. pii: genetics.115.184275. 10.1534/genetics.115.184275 PubMed 26837755
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