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SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.
Schwartz ML, Jorgensen EM
Genetics. 2016 Feb 2. pii: genetics.115.184275.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73682pMLS234SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker
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73683pMLS235SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker
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73684pMLS236SapTrap 3-site Destination vector for internal GFP tagging with embedded Cbr-unc-119 selectable marker
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73685pMLS260Punc-17::2xNLS-FLP-D5 Expression vector
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73687pMLS360Punc-47::2xNLS-FLP-D5 Expression vector
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73714pMLS134SapTrap destination vector for building sgRNA expression vectors.
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73715pMLS256SapTrap destination vector for building combined sgRNA expression + repair template vectors
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73716pMLS257SapTrap destination vector for building repair template only vectors
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73717pMLS328C. elegans germline CRE expression vector
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73718pMLS262C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector
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73719pMLS338SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9
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73720pMLS252SapTrap GFP + Cbr-unc-119 donor plasmid
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73721pMLS254SapTrap Halotag + Cbr-unc-119 donor plasmid
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73722pMLS271SapTrap SNAPtag + Cbr-unc-119 donor plasmid
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73723pMLS286Saptrap tagRFP + Cbr-unc-119 donor plasmid
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73724pMLS291SapTrap mCherry + Cbr-unc-119 donor plasmid
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73725pMLS292SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid
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73726pMLS268SapTrap SL2 C-terminal connector donor plasmid
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73727pMLS269SapTrap SL2::SV40-NLS C-terminal connector donor plasmid
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73728pMLS272SapTrap syntron-embedded FLP-on C-terminal connector donor plasmid
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73729pMLS279SapTrap FLP-on C-terminal connector donor plasmid
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73730pMLS287SapTrap flexible linker C-terminal connector donor plasmid
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73731pMLS382SapTrap Flagtag-TEV C-terminal connector donor plasmid
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73732pMLS281SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid
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73733pMLS282SapTrap FLP-on N-terminal connector donor plasmid
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73734pMLS285SapTrap egl-13 NLS connector donor plasmid
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73735pMLS288SapTrap flexible linker N-terminal connector donor plasmid
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73736pMLS383SapTrap TEV-Flagtag N-terminal connector donor plasmid
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73737pMLS297SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid
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73738pMLS280Simple cloning vector
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73739pWD509Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5
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