CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair.
Katic I, Xu L, Ciosk R
G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. PubMed Article
Katic I, Xu L, Ciosk R
G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65629 | pIK198 | "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences. |
| 65630 | pIK208 | sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)" |
| 65631 | pIK127 | Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter. |
| 65632 | pIK137 | Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment |