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CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair.
Katic I, Xu L, Ciosk R
G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
65629pIK198"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
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65630pIK208sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"
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65631pIK127Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.
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65632pIK137Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment
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