Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, Palumbo E, Ponomarenko J, Guigo R, Johnson R
PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar. PubMed
Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, Palumbo E, Ponomarenko J, Guigo R, Johnson R
PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 10.1371/journal.pcbi.1005341. eCollection 2017 Mar. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 78534 | pDECKO_mCherry | Backbone to clone single or paired sgRNAs |
| 78535 | pDECKO-mCherry GFP | Control plasmid (paired gRNAs against GFP) |
| 78536 | pDECKO-mCherry MALAT1_Enhancer.1 | Experimental plasmid |
| 78537 | pDECKO-mCherry MALAT1_Enhancer.2 | Experimental plasmid |
| 78538 | pDECKO-mCherry MALAT1_Enhancer.3 | Experimental plasmid |
| 78539 | pDECKO-mCherry MALAT1_Enhancer.4 | Experimental plasmid |
| 78540 | pDECKO-mCherry MALAT1_Exon.1 | Experimental plasmid |
| 78541 | pDECKO-mCherry MALAT1_Exon.2 | Experimental plasmid |
| 78542 | pDECKO-mCherry MALAT1_Exon.3 | Experimental plasmid |
| 78543 | pDECKO-mCherry MALAT1_Exon.4 | Experimental plasmid |
| 78544 | pDECKO-mCherry TFRC_B | Experimental plasmid |
| 78545 | pLentiCas9-BFP | Fusion protein Cas9+BFP |
| 78546 | pLentiCas9-GFP | Fusion protein Cas9+GFP |
| 78547 | pLentiCas9-T2A-BFP | Cas9 protein with BFP |
| 78548 | pLentiCas9-T2A-GFP | Cas9 protein with GFP |