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Targeting cells with single vectors using multiple-feature Boolean logic.
Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K
Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 2014 Jun 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
55632pAAV-Ef1a-mCherry-IRES-CreExpresses Cre in Mammalian Cells
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55633pAAV-EF1a-mCherry-IRES-DreExpresses Dre in Mammalian Cells
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55634pAAV-EF1a-mCherry-IRES-FlpoExpresses Flpo in Mammalian Cells
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55635pAAV-EF1a-sCreExpresses sCre in Mammalian Cells
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55636pAAV-EF1a-CreExpresses Cre in Mammalian Cells
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55637pAAV-EF1a-FlpoExpresses Flpo in Mammalian Cells
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55638pAAV-EF1a-vCreExpresses vCre in Mammalian Cells
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55639pAAV-Ef1a-fDIO hChR2(H134R)-EYFPFlp-Dependent ChR2-EYFP
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55640pAAV-Ef1a-dDIO hChR2(H134R)-EYFPDre-Dependent ChR2-EYFP
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55641pAAV-Ef1a-fDIO EYFPFlp-Dependent EYFP
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55642pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFPsCre-Dependent ChR2-EYFP
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55643pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFPvCre-Dependent ChR2-EYFP
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55644pAAV-nEF Con/Fon hChR2(H134R)-EYFPCre-on/Flp-on ChR2-EYFP under the short Ef1a promoter
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55645pAAV-hSyn Con/Fon hChR2(H134R)-EYFPCre-on/Flp-on ChR2-EYFP under the Synapsin promoter
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55646pAAV-hSyn Con/Foff hChR2(H134R)-EYFP Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter
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55647pAAV-nEF Con/Foff hChR2(H134R)-EYFPCre-on/Flp-off ChR2-EYFP under the short Ef1a promoter
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55648pAAV-hSyn Coff/Fon hChR2(H134R)-EYFPCre-off/Flp-on ChR2-EYFP under the Synapsin promoter
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55649pAAV-hnEF Coff/Fon hChR2(H134R)-EYFPCre-off/Flp-on ChR2-EYFP under the short Ef1a promoter
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55650pAAV-hSyn Con/Fon EYFPCre-on/Flp-on EYFP under the Synapsin promoter
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55651pAAV-hSyn Con/Foff EYFPCre-on/Flp-off EYFP under the Synapsin promoter
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55652pAAV-hSyn Coff/FonEYFPCre-off/Flp-on EYFP under the Synapsin promoter
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