169,450 results
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Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCMV-3XFLAG-hINO80
Plasmid#44149DepositorAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)
Viral Prep#105545-AAV8PurposeReady-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHITagsEGFPAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-pA-Hia5
Plasmid#174372PurposeSEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein A - hia5
Tags6xHis and Protein AExpressionBacterialAvailable SinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX333-T2A-EGFP
Plasmid#197417PurposeSpCas9-T2A-EGFP with dual sgRNA cloning backbonesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCBh; U6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV PHP.eB)
Viral Prep#104492-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Blasticidin
Plasmid#75085Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPGKPhiC31obpA
Plasmid#13795PurposeExpression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.DepositorInsertPhiC31o
ExpressionMammalianPromoterPGKAvailable SinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SV40 LT
Plasmid#13970DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi
Plasmid#199808Purposevector for constitutive CRISPRi-mediated gene knockdown in S. aureusDepositorInsertsdCas9
sgRNA with GFP cassette
UseCRISPRPromoterP23 and PRAB17 (sgRNA) and SarAP1 (GFP)Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR(DD)-YFP
Plasmid#29325DepositorInsertDHFR(DD)-YFP
UseRetroviralTagsHA and YFPMutationChange Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …Available SinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
ExpressionBacterialPromoterpLacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 711-1034
Plasmid#75141PurposeExpresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 711-1034PromoterCMVAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 1-90
Plasmid#75137PurposeExpresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
TagsFlagExpressionMammalianMutationfragment encoding amino acids 1-90PromoterCMVAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
p6XHis-T7(P266L)
Plasmid#174866PurposeExpresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.DepositorInsertT7 RNA polymerase
Tags6XHis-tagExpressionBacterialMutationP266LPromoterlac promoterAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
ExpressionMammalianAvailable SinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mouse Chromatin Regulator Library
Pooled Library#200011PurposeKnockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianPromoterPGKAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only