168,037 results

1. pCOLA-A-pC-tac

   Plasmid

   #213494

   Purpose

   Encodes Node A of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   rpaI (rpaI )
   tetR
   4cl
   tal
   

   Use

   Synthetic Biology

   Tags

   ASV tag and LVA tag

   Available Since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-mNeptune2-SKL

   Plasmid

   #183729

   Purpose

   For exrpression of mNeptune2 targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   mNeptube2
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSII42N-TDH3pr

   Plasmid

   #194531

   Purpose

   High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

   Depositor

   Patrick Gibney

   Article

   Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETA-qmTQ2-cAMP

   Plasmid

   #225249

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(Ct)_FLAG3

   Plasmid

   #223896

   Purpose

   4G-cloning donor for C-terminal FLAG3 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   FLAG3
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPPI4-SNAP-CTLA-4-His6

   Plasmid

   #223584

   Purpose

   Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Tags

   His6 and SNAP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-B-pC

   Plasmid

   #213495

   Purpose

   Encodes Node B of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   cinI (cinI )
   lacI (lacI )
   lambda cI (lambdap50 )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   ASV tag and LVA tag

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCC1R

   Plasmid

   #213497

   Purpose

   Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   pcaU
   nahR
   rpaR (rpaR )
   phlF
   cinR
   vanR
   aiiA
   

   Use

   Synthetic Biology

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-C

    Plasmid

    #213496

    Purpose

    Encodes Node C of the three-node Turing circuit

    Depositor

    Mark Isalan

    Inserts

    lambda cI 5G6GP
    mCherry
    

    Use

    Synthetic Biology

    Tags

    ASV tag and LVA tag

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYSD100

    Plasmid

    #212924

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-NRF2-E79Q-V5-miniTurbo

    Plasmid

    #207814

    Purpose

    C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    NRF2-E79Q-V5-miniTurbo (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Mutation

    E79Q

    Promoter

    UBC

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. T1B/pUBQ10(mTurq->mScarlet)

    Plasmid

    #195946

    Purpose

    Bxb1 target with pUBQ10 switching from mTurq to mScarlet

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
    

    Expression

    Plant

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. L1B1

    Plasmid

    #195944

    Purpose

    Level 1 Bxb1 cassette with constitutive promoter : pPP2AA3-Bxb1-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pPP2AA3-Bxb1-tUBQ1
    

    Expression

    Plant

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Anti-VAChT [N425/45]

    Plasmid

    #190326

    Purpose

    Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9_CL

    Plasmid

    #166245

    Purpose

    regulated dCas9 generator

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    P112-sgRNA-term-P104-RBS1-dCas9-B15
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and Ec-TTL-P104

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

    Plasmid

    #118807

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of NTD in full-length TDP43

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. 4QOX

    Plasmid

    #61959

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-CDPK4
    

    Use

    Baculovirus expression

    Tags

    MHHHHHHSSGRENLYFQG

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available Since

    May 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. RP185 pUAST EGFP:Or47b

    Plasmid

    #59525

    Purpose

    N-terminal fusion of GFP to Drosophila melanogaster Or47b

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or47b (Or47b Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

20. RP132 pUAST EGFP:Or43a

    Plasmid

    #59523

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Or43a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or43a (Or43a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only