TULIPs: tunable, light-controlled interacting protein tags for cell biology.
Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M
Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. PubMed Article
Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M
Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 34966 | pDS240 | Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope). |
| 34967 | pDS247 | |
| 34968 | pDS248 | |
| 34969 | pDS250 | |
| 34970 | pDS255 | |
| 34971 | pDS257 | Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep. |
| 34972 | pDS275 | Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations). |
| 34973 | pDS277 | Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation). |
| 34974 | pDS271 | |
| 34975 | pDS272 | |
| 34977 | pDS190 | |
| 34978 | pDS191 | |
| 34980 | pDS220 | Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep. |
| 34981 | pDS221 | Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep. |
| 34982 | pDS244 | |
| 34983 | pDS282 | |
| 34984 | pDS295 | |
| 34985 | pDS296 | |
| 34986 | pDS297 |