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(Plasmid #34966)


Item Catalog # Description Quantity Price (USD)
Plasmid 34966 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4263
  • Modifications to backbone
    PGAL-Mid2(SS/TM)-GFP-LOVpep-TCYC1 cassette inserted into YIPlac128
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Constitutively active LOVpep
  • Species
    S. cerevisiae (budding yeast); A. sativa
  • Insert Size (bp)
  • Mutation
    K533∆, high-affinity epitope
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SphI (destroyed during cloning)
  • 5′ sequencing primer M13 fwd
  • 3′ sequencing primer M13 rev
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GFP(S65T) CDS and other sequences from pGREG plasmids (EUROSCARF). A. sativa LOV2 CDS from Kevin Gardner, UT Southwestern Medical Center

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS240 was a gift from Michael Glotzer (Addgene plasmid # 34966 ; ; RRID:Addgene_34966)
  • For your References section:

    TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287