|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34966||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4263
Modifications to backbonePGAL-Mid2(SS/TM)-GFP-LOVpep-TCYC1 cassette inserted into YIPlac128
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameConstitutively active LOVpep
SpeciesS. cerevisiae (budding yeast); A. sativa
Insert Size (bp)2229
MutationK533∆, high-affinity epitope
- Promoter GAL1
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site SphI (destroyed during cloning)
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The LOV2 domain is amino acids 404-540 and differs from the wild-type A. sativa LOV2 sequence by the point mutations G528A and N534E. See Strickland et al. for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDS240 was a gift from Michael Glotzer (Addgene plasmid # 34966 ; http://n2t.net/addgene:34966 ; RRID:Addgene_34966)
For your References section:TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287