|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||34983||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backbonePTEF–cpPDZ–mCherry–TCYC1 cassette inserted into YIPlac211
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
- Promoter TEF
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySequences from pGREG plasmids (EUROSCARF). cpPDZ CDS from Shohei Koide, University of Chicago
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDS282 was a gift from Michael Glotzer (Addgene plasmid # 34983 ; http://n2t.net/addgene:34983 ; RRID:Addgene_34983)
For your References section:TULIPs: tunable, light-controlled interacting protein tags for cell biology. Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M. Nat Methods. 2012 Mar 4. doi: 10.1038/nmeth.1904. 10.1038/nmeth.1904 PubMed 22388287