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GFP-wGBD
(Plasmid #26734)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26734 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; reporter for Cdc42

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Neuronal Wiskott-Aldrich Syndrome protein
  • Alt name
    N-WASP
  • Alt name
    wGBD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    276
  • Mutation
    Cdc42 binding domain of N-WASP
  • Entrez Gene
    WASL (a.k.a. N-WASP, NWASP, WASPB)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspEI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The Cdc42-binding domain of N-WASP was fused to GFP to visualize active Cdc42

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-wGBD was a gift from William Bement (Addgene plasmid # 26734 ; http://n2t.net/addgene:26734 ; RRID:Addgene_26734)
  • For your References section:

    Concentric zones of active RhoA and Cdc42 around single cell wounds. Benink HA, Bement WM. J Cell Biol. 2005 Jan 31. 168(3):429-39. 10.1083/jcb.200411109 PubMed 15684032