|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26737||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4097
Vector typeMammalian Expression ; reporter for F-actin
Growth in Bacteria
Copy numberHigh Copy
MutationCalponin homology domain of utrophin (aa# 1-261). See depositor comments below
- Promoter CMV IE94
/ Fusion Protein
- GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
The calponin homology domain of utrophin (Utr-CH) was fused to eGFP in order to visualize F-actin.
Uploaded sequence from depositing lab includes the complete GFP-utrophin sequence.
Please note that mutation Q236R in utrophin was found during Addgene's quality control. The mutation does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GFP-UtrCH was a gift from William Bement (Addgene plasmid # 26737 ; http://n2t.net/addgene:26737 ; RRID:Addgene_26737)
For your References section:Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Burkel BM, von Dassow G, Bement WM. Cell Motil Cytoskeleton. 2007 Nov . 64(11):822-32. 10.1002/cm.20226 PubMed 17685442