|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26742||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4097
Vector typeMammalian Expression ; probe for microtubules
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)816
MutationMicrotubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882)
Entrez GeneMAP7 (a.k.a. E-MAP-115, EMAP115)
/ Fusion Proteins
- 2x mCherry (N terminal on backbone)
- Myc (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byA EMTB construct was a gift from Chloë Bulinski (Columbia University)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note: It is difficult to sequence entirely through 2 tandem mCherrys. Users are encouraged to perform a double-digest on this plasmid with BamHI and EcoRI to verify the presence of a ~2.2kb band corresponding to the EMTB insert + 2XmCherry. Please see above attachment.
The microtubule binding domain of ensconsin (EMTB) was fused to multiple mCherry molecules in order to visualize microtubules. Please note that this plasmid has recombined and is currently only 2X mCherry.
mCherry sequences have point mutation at R154 (CGG to AGA) for optimal codon usage in Xenopus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EMTB-mCherry was a gift from William Bement (Addgene plasmid # 26742 ; http://n2t.net/addgene:26742 ; RRID:Addgene_26742)
For your References section:Regulation of cytokinesis by Rho GTPase flux. Miller AL, Bement WM. Nat Cell Biol. 2009 Jan . 11(1):71-7. 10.1038/ncb1814 PubMed 19060892