Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #26742)


Item Catalog # Description Quantity Price (USD)
Plasmid 26742 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; probe for microtubules

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882)
  • GenBank ID
  • Entrez Gene
    MAP7 (a.k.a. E-MAP-115, EMAP115)
  • Tags / Fusion Proteins
    • 2x mCherry (N terminal on backbone)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: It is difficult to sequence entirely through 2 tandem mCherrys. Users are encouraged to perform a double-digest on this plasmid with BamHI and EcoRI to verify the presence of a ~2.2kb band corresponding to the EMTB insert + 2XmCherry. Please see above attachment.

The microtubule binding domain of ensconsin (EMTB) was fused to multiple mCherry molecules in order to visualize microtubules. Please note that this plasmid has recombined and is currently only 2X mCherry.

mCherry sequences have point mutation at R154 (CGG to AGA) for optimal codon usage in Xenopus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EMTB-mCherry was a gift from William Bement (Addgene plasmid # 26742 ; ; RRID:Addgene_26742)
  • For your References section:

    Regulation of cytokinesis by Rho GTPase flux. Miller AL, Bement WM. Nat Cell Biol. 2009 Jan . 11(1):71-7. 10.1038/ncb1814 PubMed 19060892