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(Plasmid #26741)


Item Catalog # Description Quantity Price (USD)
Plasmid 26741 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; probe for microtubules

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Only contains the microtubule binding-domain of ensconsin (amino acids 18-283 of GenBank reference sequence X73882)
  • GenBank ID
  • Entrez Gene
    MAP7 (a.k.a. E-MAP-115, EMAP115)
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • 3x EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A EMTB construct was a gift from Chloë Bulinski (Columbia University)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The microtubule binding domain of ensconsin (EMTB) was fused to 3 GFP molecules in order to visualize microtubules. For cloning details see Faire et al., 1999 Journal
of Cell Science 112, 4243-4255.

Note: It is difficult to sequence entirely through 3 tandem GFPs. Users are encouraged to perform a double-digest on this plasmid with EcoRI and XhoI to verify the presence of a ~3kb band corresponding to the EMTB insert + 3GFPs. Please see above attachment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EMTB-3XGFP was a gift from William Bement (Addgene plasmid # 26741 ; ; RRID:Addgene_26741)
  • For your References section:

    Regulation of cytokinesis by Rho GTPase flux. Miller AL, Bement WM. Nat Cell Biol. 2009 Jan . 11(1):71-7. 10.1038/ncb1814 PubMed 19060892