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GFP-rGBD
(Plasmid #26732)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26732 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4097
  • Vector type
    Mammalian Expression ; reporter for Rho

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rhotekin
  • Alt name
    rGBD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    249
  • Mutation
    RhoA binding domain of rhotekin
  • Entrez Gene
    rtkn2 (a.k.a. plekhk1)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The RhoA-binding domain of rhotekin (rGBD) was fused to eGFP in order to visualize active RhoA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-rGBD was a gift from William Bement (Addgene plasmid # 26732 ; http://n2t.net/addgene:26732 ; RRID:Addgene_26732)
  • For your References section:

    Concentric zones of active RhoA and Cdc42 around single cell wounds. Benink HA, Bement WM. J Cell Biol. 2005 Jan 31. 168(3):429-39. 10.1083/jcb.200411109 PubMed 15684032