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Optical Pooled Screens in Human Cells.
Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC
Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
127168LentiGuide-BC-startEntry plasmid for two-step guide-barcode cloning
127169LentiGuide-BC-CMV-PuroPCR template for CMV-puro resistance cassette
127170LentiGuide-BC-EF1a-PuroPCR template for EF1a-puro resistance cassette
127171pL_FR_HygroFrameshift reporter (control plasmid for screening)
127172p65-mNeonGreenFluorescently tagged p65 reporter
127173CROPseq-Guide-ZeoEntry plasmid for guide cloning
127458CROPseq-puro-v2Entry plasmid for guide cloning

Antibodies from Article