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pET21b(+)-Is-PETase-W159H-S238F
(Plasmid #112203)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 112203 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-21b(+)
  • Backbone manufacturer
    Novagen/Millipore Sigma
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 6237
  • Modifications to backbone
    His tag: CACCACCATCACCACCAC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
  • Species
    Ideonella sakaiensis
  • Mutation
    W159H, S238F
  • GenBank ID
    GAP38373.1
  • Promoter N/A
  • Tag / Fusion Protein
    • 6XHIS (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b(+)-Is-PETase-W159H-S238F was a gift from Gregg Beckham & Christopher Johnson (Addgene plasmid # 112203 ; http://n2t.net/addgene:112203 ; RRID:Addgene_112203)
  • For your References section:

    Characterization and engineering of a plastic-degrading aromatic polyesterase. Austin HP, Allen MD, Donohoe BS, Rorrer NA, Kearns FL, Silveira RL, Pollard BC, Dominick G, Duman R, El Omari K, Mykhaylyk V, Wagner A, Michener WE, Amore A, Skaf MS, Crowley MF, Thorne AW, Johnson CW, Woodcock HL, McGeehan JE, Beckham GT. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4350-E4357. doi: 10.1073/pnas.1718804115. Epub 2018 Apr 17. 10.1073/pnas.1718804115 PubMed 29666242