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RNA targeting with CRISPR-Cas13.
Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F
Nature. 2017 Oct 4. doi: 10.1038/nature24049.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
91902pC014 - LwCas13a-msfGFPExpresses active LwCas13a for mammalian RNA targeting
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91905pC015 - dLwCas13a-NFExpresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
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91906pC016 - LwCas13a guide expression backbone with U6 promoterBackbone for cloning LwCas13a guides under U6 promoter
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91907pC017 - LwCas13a guide expression backbone with tRNAval promoterBackbone for cloning LwCas13a guides under tRNAval promoter
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91908pC018 - LshCas13a from Leptotrichia shahiiExpresses LshCas13a for bacterial expression and contains backbone for spacer cloning
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91909pC019 - LwCas13a from Leptotrichia wadeiExpresses LwCas13a for bacterial expression and contains backbone for spacer cloning
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91910pC020 - LseCas13a from Listeria seeligeriExpresses LseCas13a for bacterial expression and contains backbone for spacer cloning
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91911pC021 - LbmCas13a from Lachnospiraceae bacterium MA2020Expresses LbmCas13a for bacterial expression and contains backbone for spacer cloning
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91912pC022 - LbnCas13a from Lachnospiraceae bacterium NK4A179Expresses LbnCas13a for bacterial expression and contains backbone for spacer cloning
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91913pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning
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91914pC024 - CgCas13a from Carnobacterium gallinarum DSM 4847Expresses CgCas13a for bacterial expression and contains backbone for spacer cloning
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91915pC025 - Cg2Cas13a from Carnobacterium gallinarum DSM 4847Expresses Cg2Cas13a for bacterial expression and contains backbone for spacer cloning
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91916pC026 - PpCas13a from Paludibacter propionicigenes WB4Expresses PpCas13a for bacterial expression and contains backbone for spacer cloning
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91917pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning
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91918pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning
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91919pC029 - Lw2Cas13a from Leptotrichia wadei F0279Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning
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91920pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning
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91921pC031 - RcrCas13a from Rhodobacter capsulatus R121Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning
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91922pC032 - RcdCas13a from Rhodobacter capsulatus DE442Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning
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91924pC034 - LwCas13a-msfGFP-2A-BlastExpresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker
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91925pC035 - dLwCas13a-msfGFPExpresses catalytically inactive LwCas13a for mammalian RNA targeting
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107691pJJB29635SPromoter-LwaCas13a-HSPTerminator
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107694pJJB302OsActin Promoter-LwaCas13a-HSP Terminator
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107699pJJB308AtU6 Promoter-LwaCas13a-gRNA Scaffold
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