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pC015 - dLwCas13a-NF
(Plasmid #91905)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 91905 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 5876
  • Total vector size (bp) 10622
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dLwCas13a-eGFP-ZF-KRAB
  • Alt name
    dead LwCas13a
  • Alt name
    dead LwC2c2
  • Alt name
    dCas13a
  • Species
    H. sapiens (human); Leptotrichia wadei
  • Insert Size (bp)
    4806
  • Mutation
    R474A and R1046A
  • Promoter CAG
  • Tags / Fusion Proteins
    • eGFP (C terminal on insert)
    • ZF CCR5TC (C terminal on insert)
    • KRAB-A (C terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC015 - dLwCas13a-NF was a gift from Feng Zhang (Addgene plasmid # 91905)
  • For your References section:

    RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959