pC016 - LwCas13a guide expression backbone with U6 promoter
PurposeBackbone for cloning LwCas13a guides under U6 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||91906||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||91906-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2850
- Total vector size (bp) 2908
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameLwCas13a Guide site
Insert Size (bp)58
Information for Cloning Grade DNA (Catalog # 91906-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC016 - LwCas13a guide expression backbone with U6 promoter was a gift from Feng Zhang (Addgene plasmid # 91906 ; http://n2t.net/addgene:91906 ; RRID:Addgene_91906)
For your References section:RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959