pC0043-PspCas13b crRNA backbone
PurposeFor cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||103854||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
|Cloning Grade DNA||103854-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95 *|
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- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 2962
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameU6-PspCas13b DR-BbsI-BbsI-polyT
Insert Size (bp)314
Benchling link: https://benchling.com/s/seq-OH6nMmZCZn930BWqcFNa
Information for Cloning Grade DNA (Catalog # 103854-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pC0043-PspCas13b crRNA backbone was a gift from Feng Zhang (Addgene plasmid # 103854 ; http://n2t.net/addgene:103854 ; RRID:Addgene_103854)
For your References section:RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703