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pC0043-PspCas13b crRNA backbone Citations (26)

Originally described in: RNA editing with CRISPR-Cas13.
Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
PubMed Journal

Articles Citing pC0043-PspCas13b crRNA backbone

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. PubMed

Associated Plasmids

Programmable Inhibition and Detection of RNA Viruses Using Cas13. Freije CA, Myhrvold C, Boehm CK, Lin AE, Welch NL, Carter A, Metsky HC, Luo CY, Abudayyeh OO, Gootenberg JS, Yozwiak NL, Zhang F, Sabeti PC. Mol Cell. 2019 Dec 5;76(5):826-837.e11. doi: 10.1016/j.molcel.2019.09.013. Epub 2019 Oct 10. PubMed
Targeted mRNA demethylation using an engineered dCas13b-ALKBH5 fusion protein. Li J, Chen Z, Chen F, Xie G, Ling Y, Peng Y, Lin Y, Luo N, Chiang CM, Wang H. Nucleic Acids Res. 2020 Jun 4;48(10):5684-5694. doi: 10.1093/nar/gkaa269. PubMed
N(6)-methyladenosine regulates glycolysis of cancer cells through PDK4. Li Z, Peng Y, Li J, Chen Z, Chen F, Tu J, Lin S, Wang H. Nat Commun. 2020 May 22;11(1):2578. doi: 10.1038/s41467-020-16306-5. PubMed
Massively parallel Cas13 screens reveal principles for guide RNA design. Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana NE. Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 10.1038/s41587-020-0456-9. Epub 2020 Mar 16. PubMed

Associated Plasmids

Abrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells. Cui J, Techakriengkrai N, Nedumpun T, Suradhat S. Sci Rep. 2020 Jun 15;10(1):9617. doi: 10.1038/s41598-020-66775-3. PubMed
Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Wilson C, Chen PJ, Miao Z, Liu DR. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6. PubMed

Associated Plasmids

TRADES: Targeted RNA Demethylation by SunTag System. Mo J, Chen Z, Qin S, Li S, Liu C, Zhang L, Ran R, Kong Y, Wang F, Liu S, Zhou Y, Zhang X, Weng X, Zhou X. Adv Sci (Weinh). 2020 Aug 16;7(19):2001402. doi: 10.1002/advs.202001402. eCollection 2020 Oct. PubMed
Potent programmable antiviral against dengue virus in primary human cells by Cas13b RNP with short spacer and delivery by VLP. Singsuksawat E, Onnome S, Posiri P, Suphatrakul A, Srisuk N, Nantachokchawapan R, Praneechit H, Sae-Kow C, Chidpratum P, Sa-Ngiamsuntorn K, Hongeng S, Avirutnan P, Duangchinda T, Siridechadilok B. Mol Ther Methods Clin Dev. 2021 Jun 11;21:729-740. doi: 10.1016/j.omtm.2021.04.014. Epub 2021 May 1. PubMed
In vivo discovery of RNA proximal proteins via proximity-dependent biotinylation. Lin X, Fonseca MAS, Breunig JJ, Corona RI, Lawrenson K. RNA Biol. 2021 May 19:1-15. doi: 10.1080/15476286.2021.1917215. PubMed
Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance. Fareh M, Zhao W, Hu W, Casan JML, Kumar A, Symons J, Zerbato JM, Fong D, Voskoboinik I, Ekert PG, Rudraraju R, Purcell DFJ, Lewin SR, Trapani JA. Nat Commun. 2021 Jul 13;12(1):4270. doi: 10.1038/s41467-021-24577-9. PubMed

Associated Plasmids

Regulation of telomere homeostasis and genomic stability in cancer by N (6)-adenosine methylation (m(6)A). Lee JH, Hong J, Zhang Z, de la Pena Avalos B, Proietti CJ, Deamicis AR, Guzman G P, Lam HM, Garcia J, Roudier MP, Sisk AE, De La Rosa R, Vu K, Yang M, Liao Y, Scheirer J, Pechacek D, Yadav P, Rao MK, Zheng S, Johnson-Pais TL, Leach RJ, Elizalde PV, Dray E, Xu K. Sci Adv. 2021 Jul 28;7(31). pii: 7/31/eabg7073. doi: 10.1126/sciadv.abg7073. Print 2021 Jul. PubMed
Developing PspCas13b-based enhanced RESCUE system, eRESCUE, with efficient RNA base editing. Li G, Wang Y, Li X, Wang Y, Huang X, Gao J, Hu X. Cell Commun Signal. 2021 Aug 11;19(1):84. doi: 10.1186/s12964-021-00716-z. PubMed
Compact RNA editors with small Cas13 proteins. Kannan S, Altae-Tran H, Jin X, Madigan VJ, Oshiro R, Makarova KS, Koonin EV, Zhang F. Nat Biotechnol. 2021 Aug 30. pii: 10.1038/s41587-021-01030-2. doi: 10.1038/s41587-021-01030-2. PubMed

Associated Plasmids

Targeted inhibition of Zika virus infection in human cells by CRISPR-Cas13b. Chen P, Chen M, Chen Y, Jing X, Zhang N, Zhou X, Li X, Long G, Hao P. Virus Res. 2022 Apr 15;312:198707. doi: 10.1016/j.virusres.2022.198707. Epub 2022 Feb 9. PubMed
CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells. Xu M, Chigumira T, Chen Z, Tones J, Zhao R, Dahl KN, Chenoweth DM, Zhang H. Front Mol Biosci. 2022 Jan 31;8:785160. doi: 10.3389/fmolb.2021.785160. eCollection 2021. PubMed
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells. Ai Y, Liang D, Wilusz JE. Nucleic Acids Res. 2022 Mar 4. pii: 6542487. doi: 10.1093/nar/gkac159. PubMed

Associated Plasmids

Enhancer RNA Transcription Is Essential for a Novel CSF1 Enhancer in Triple-Negative Breast Cancer. Lewis MW, Wisniewska K, King CM, Li S, Coffey A, Kelly MR, Regner MJ, Franco HL. Cancers (Basel). 2022 Apr 6;14(7). pii: cancers14071852. doi: 10.3390/cancers14071852. PubMed
Inducible and reversible RNA N(6)-methyladenosine editing. Shi H, Xu Y, Tian N, Yang M, Liang FS. Nat Commun. 2022 Apr 12;13(1):1958. doi: 10.1038/s41467-022-29665-y. PubMed
N6-methyladenosine regulated FGFR4 attenuates ferroptotic cell death in recalcitrant HER2-positive breast cancer. Zou Y, Zheng S, Xie X, Ye F, Hu X, Tian Z, Yan SM, Yang L, Kong Y, Tang Y, Tian W, Xie J, Deng X, Zeng Y, Chen ZS, Tang H, Xie X. Nat Commun. 2022 May 13;13(1):2672. doi: 10.1038/s41467-022-30217-7. PubMed
Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13. Burris BJD, Molina Vargas AM, Park BJ, O'Connell MR. Methods. 2022 Oct;206:58-68. doi: 10.1016/j.ymeth.2022.08.007. Epub 2022 Aug 17. PubMed
Genetically encoded chemical crosslinking of RNA in vivo. Sun W, Wang N, Liu H, Yu B, Jin L, Ren X, Shen Y, Wang L. Nat Chem. 2023 Jan;15(1):21-32. doi: 10.1038/s41557-022-01038-4. Epub 2022 Oct 6. PubMed
Pseudoknot-targeting Cas13b combats SARS-CoV-2 infection by suppressing viral replication. Yu D, Han HJ, Yu J, Kim J, Lee GH, Yang JH, Song BM, Tark D, Choi BS, Kang SM, Heo WD. Mol Ther. 2023 Jun 7;31(6):1675-1687. doi: 10.1016/j.ymthe.2023.03.018. Epub 2023 Mar 21. PubMed
CRISPR Based Programmable RNA Editing in Primary Hippocampal Neurons. Ravichandran K, Khargonkar T, Samaddar S, Banerjee S. Curr Protoc. 2023 Sep;3(9):e886. doi: 10.1002/cpz1.886. PubMed
Sex-differences in proteasome-dependent K48-polyubiquitin signaling in the amygdala are developmentally regulated in rats. Farrell K, Auerbach A, Liu C, Martin K, Pareno M, Ray WK, Helm RF, Biase F, Jarome TJ. Biol Sex Differ. 2023 Nov 10;14(1):80. doi: 10.1186/s13293-023-00566-z. PubMed
Cas13b-mediated RNA targeted therapy alleviates genetic dilated cardiomyopathy in mice. Li J, Xuan H, Kuang X, Li Y, Lian H, Yu N. Cell Biosci. 2024 Jan 4;14(1):4. doi: 10.1186/s13578-023-01143-y. PubMed

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