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Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
109049pXR001: EF1a-CasRx-2A-EGFPCasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting
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109050pXR002: EF1a-dCasRx-2A-EGFPCatalytically inactive dCasRx with 2A-EGFP
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109053pXR003: CasRx gRNA cloning backbonehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.
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109054pXR004: CasRx pre-gRNA cloning backbonehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs
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