pXR004: CasRx pre-gRNA cloning backbone
Purpose(Empty Backbone) hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||109054||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||109054-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2686
Modifications to backboneinserted gRNA expression cassette
Vector typeMammalian Expression, CRISPR
- Promoter hU6
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Information for Cloning Grade DNA (Catalog # 109054-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXR004: CasRx pre-gRNA cloning backbone was a gift from Patrick Hsu (Addgene plasmid # 109054 ; http://n2t.net/addgene:109054 ; RRID:Addgene_109054)
For your References section:Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033. 10.1016/j.cell.2018.02.033 PubMed 29551272