pXR002: EF1a-dCasRx-2A-EGFP
(Plasmid #109050)

• Purpose: Catalytically inactive dCasRx with 2A-EGFP
• Depositing Lab: Patrick Hsu
• Publication: Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.

Backbone

• Vector backbone: lenti(AMP)
  (Search Vector Database)
• Total vector size (bp): 12990
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCasRx
• Alt name: NLS-dRfxCas13d-NLS
• Species: H. sapiens (human); Ruminococcus flavefaciens XPD3002
• Insert Size (bp): 3000
• Mutation: R239A/H244A/R858A/H863A
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • HA (C terminal on insert)
  • 2A-eGFP (C terminal on backbone)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Institut Pasteur Label License for cPPT
• Industry Terms:
  • Not Available to Industry