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pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
(Plasmid #103865)


Item Catalog # Description Quantity Price (USD)
Plasmid 103865 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 9360
  • Total vector size (bp) 12606
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Prevotella sp. P5-125
  • Insert Size (bp)
  • Mutation
    Contains the H133A and H1058A mutations which inactivate the HEPN RNase domains of Cas13b.
  • GenBank ID
  • Promoter EF1a
  • Tags / Fusion Proteins
    • HIV NES (C terminal on insert)
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A was a gift from Feng Zhang (Addgene plasmid # 103865 ; ; RRID:Addgene_103865)
  • For your References section:

    RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703