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pCMV-dCas13-inactive M3nls
(Plasmid #157854)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 157854 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas13-inactive dCas13-inactive M3nls
  • Species
    Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens
  • Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
  • Entrez Gene
    METTL3 (a.k.a. IME4, M6A, MT-A70, Spo8, hMETTL3)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-dCas13-inactive M3nls was a gift from David Liu (Addgene plasmid # 157854 ; http://n2t.net/addgene:157854 ; RRID:Addgene_157854)
  • For your References section:

    Programmable m(6)A modification of cellular RNAs with a Cas13-directed methyltransferase. Wilson C, Chen PJ, Miao Z, Liu DR. Nat Biotechnol. 2020 Jun 29. pii: 10.1038/s41587-020-0572-6. doi: 10.1038/s41587-020-0572-6. 10.1038/s41587-020-0572-6 PubMed 32601430