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Compact RNA editors with small Cas13 proteins.
Kannan S, Altae-Tran H, Jin X, Madigan VJ, Oshiro R, Makarova KS, Koonin EV, Zhang F
Nat Biotechnol. 2021 Aug 30. pii: 10.1038/s41587-021-01030-2. doi: 10.1038/s41587-021-01030-2. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176314pAB1619 hU6-BpiI-Cas13bt1-DRhU6-BpiI-Cas13bt1-DR (crRNA expression)
176315pAB1620 hU6-BpiI-Cas13bt3-DRhU6-BpiI-Cas13bt3-DR (crRNA expression)
176316pAB1678 CMV-HIVNES-GS-Cas13bt1CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)
176317pAB1679 CMV-HIVNES-GS-Cas13bt3CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)
176319pAB1676 REPAIR.t1CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
176320pAB1677 REPAIR.t3CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
176321pAB1923 REPAIR.t1-SCMV-HIVNES-GS-dCas13bt1- (GGS)2- huADAR2dd(E488Q/E620G/Q696L) (REPAIR.t1-S expression, specificity enhancing mutations)
176323pAB2076 pAAV REPAIR.t1pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

Antibodies from Article