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(Plasmid #103870)


Item Catalog # Description Quantity Price (USD)
Plasmid 103870 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 9846
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    Prevotella sp. P5-125
  • Insert Size (bp)
  • Mutation
    Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity.
  • GenBank ID
  • Tag / Fusion Protein
    • HIV NES (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    human ADAR2 deaminase domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Only the deaminase domain (AA 276-702 are expressed). Glutamate 488 is mutated to glutamine. Threonine 375 is mutated to glycine to enhance specificity of deaminase reaction.
  • Entrez Gene
  • Tag / Fusion Protein
    • GSGGGGS (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Benchling link:

Addgene's quality control sequencing finds an additional P691S amino acid residue substitution, but this residue substitution is not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G) was a gift from Feng Zhang (Addgene plasmid # 103870 ; ; RRID:Addgene_103870)
  • For your References section:

    RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703