Purpose(Empty Backbone) AtU6 Promoter-LwaCas13a-gRNA Scaffold
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107699||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Unknown
- 5′ sequencing primer GGAAGAGCGCCCACCTGC
- 3′ sequencing primer CAAATAGGGGTTCATCGCAC (Common Sequencing Primers)
This is a MOD_B plasmid compatible with the Cermek et al. toolkit.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJJB308 was a gift from Daniel Voytas (Addgene plasmid # 107699 ; http://n2t.net/addgene:107699 ; RRID:Addgene_107699)
For your References section:RNA targeting with CRISPR-Cas13. Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, Voytas DF, Ting AY, Zhang F. Nature. 2017 Oct 4. doi: 10.1038/nature24049. 10.1038/nature24049 PubMed 28976959