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pCS2-MT GLI2 delta N
(Plasmid #17649)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17649 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLI2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3800
  • Mutation
    Missing 328 N-terminal amino acids
  • Entrez Gene
    GLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)
  • Tag / Fusion Protein
    • 6x Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-MT GLI2 delta N was a gift from Erich Roessler (Addgene plasmid # 17649 ; http://n2t.net/addgene:17649 ; RRID:Addgene_17649)
  • For your References section:

    A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Roessler E, Ermilov AN, Grange DK, Wang A, Grachtchouk M, Dlugosz AA, Muenke M. Hum Mol Genet. 2005 Aug 1. 14(15):2181-8. 10.1093/hmg/ddi222 PubMed 15994174