|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16419||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameglioma-associated oncogene homolog 1
SpeciesH. sapiens (human)
Insert Size (bp)3601
Entrez GeneGLI1 (a.k.a. GLI, PAPA8, PPD1)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GLI K12 was a gift from Bert Vogelstein (Addgene plasmid # 16419 ; http://n2t.net/addgene:16419 ; RRID:Addgene_16419)
For your References section:The GLI gene is a member of the Kruppel family of zinc finger proteins. Kinzler KW, Ruppert JM, Bigner SH, Vogelstein B. Nature. 1988 Mar 24. 332(6162):371-4. 10.1038/332371a0 PubMed 2832761