pCS2 mt-GFP (BamHI minus)
(Plasmid #15681)

• Purpose: (Empty Backbone)
• Depositing Lab: Michael Klymkowsky
• Publication: GFP plasmids (unpublished)

Backbone

• Vector backbone: pCS2 mt-GFP
• Backbone size (bp): 5100
• Vector type: xenopus/mammalian/avian/zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • 6x Myc (N terminal on backbone)
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pCS2 plasmid can be used to synthesize RNA via its SP6 promoter, or directly drive expression in eukarotic cells via the CMV promoter.

In pCS2mt-GFP, the S65T mutant form of GFP has been inserted such that the myc tags and the GFP are in-frame.

You can subclone your favorite sequence between the myc tags and the GFP using EcoRI and Xba I sites. The EcoRI site's AAT defines the reading frame for the insert. This produces an N-terminally myc-tagged, C-terminally GFP-tagged chimeric polypeptide.

Please see lab website for more information (go to Methods -> Green Plasmids): http://spot.colorado.edu/~klym/